Fusion protein of CD137 antibody and CD40L and application thereof

The present application provides a fusion protein comprising a Fab fragment capable of specifically binding to a 4-1BB molecule, a method for preparing such a fusion protein, and uses thereof; the CD40L can be specifically combined with a CD40 molecule, and the N end of the first CD40L is connected with the C end of the light chain or the heavy chain of the Fab fragment through a first peptide linker. The fusion protein of the CD137 antibody and the CD40L can be specifically combined with CD40, has the effects of inducing dendritic cells to mature, activating lymphocytes and the like, and can be used for treating tumors and immune related diseases; meanwhile, the fusion protein of the CD137 antibody and the CD40L can target the 4-1BB and activate the signal transduction pathway of the 4-1BB, so that the immune response is enhanced. 本申请提供了一种融合蛋白，用于制备此类融合蛋白的方法及其用途，所述融合蛋白包含能特异性结合4-1BB分子的Fab片段；和能特异性结合CD40分子的CD40L，所述第一CD40L的N端通过第一肽接头与所述Fab片段的轻链或重链的C端连接。本申请的CD137抗体和CD40L的融合蛋白能够与CD40特异性结合，具有诱导树突状细胞成熟、活化淋巴细胞等效果，可用于治疗