Analysis and screening method of local chicken breed quality characteristic related genes

The invention provides a method for analyzing and screening related genes of breeder quality characteristics of local chickens, which comprises the following steps: selecting adult chicken samples of different populations, establishing sample information, collecting blood samples, and storing for la...

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Hauptverfasser: XU DAN, ZHAO XIAOLING, TIAN YAOFU, WU YOUHAO, MIAO XIAOMENG, TANG JIGAO, FENG YULONG, DAI GUOTAO, WEI SHUO, HAN XUE, SHU GANG, WU GEMIN, LI LIANG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a method for analyzing and screening related genes of breeder quality characteristics of local chickens, which comprises the following steps: selecting adult chicken samples of different populations, establishing sample information, collecting blood samples, and storing for later use; extracting DNA from the blood sample, then carrying out whole genome re-sequencing, and filtering data; performing comparison and variation detection on the filtered re-sequencing data and a chicken reference genome to obtain SNP data information; 4 algorithms of Fst, pi-ratio, XP-CLR and XP-EHH are combined, the obtained SNP data information is subjected to selective elimination analysis, and candidate regions of germplasm characteristic related genes are obtained; and performing function enrichment analysis on the genes in the candidate region, and obtaining candidate genes related to germplasm characteristics of different populations by combining phenotypic germplasm characteristics of different varieti