Preparation method of electrochemical sensor for detecting escherichia coli

The invention discloses a preparation method of an electrochemical sensor for detecting escherichia coli. The preparation method comprises the following steps: (1) preparing a CFGO/CB compound; (2) activating and washing; (3) adding a bacteriophage EP01 suspension, reacting, centrifuging, and washin...

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Bibliographische Detailangaben
Hauptverfasser: MO YUPENG, HUANG JIJIE, ZENG FANBAO, WANG XIAOYE, ZHOU YUQING, HUANG WEIQIAN, LI XUN, LI ZIYONG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a preparation method of an electrochemical sensor for detecting escherichia coli. The preparation method comprises the following steps: (1) preparing a CFGO/CB compound; (2) activating and washing; (3) adding a bacteriophage EP01 suspension, reacting, centrifuging, and washing, so as to obtain CFGO-CB-EP01; re-suspending is carried out; (4) dropwise adding the mixed solution to a working area of a bare glassy carbon electrode, and drying to obtain an EP01-CFGO/CB/GCE electrode; and (5) placing in bovine serum albumin, sealing the modified electrode, and cleaning to obtain the electrochemical sensor for detecting escherichia coli. According to the electrochemical sensor constructed by the method disclosed by the invention, the electrochemical sensor for detecting the escherichia coli has a good linear relation in the escherichia coli concentration range of 1 * 10 < 2 >-1 * 10 < 7 > CFU/mL, the whole detection process can be completed within 30 minutes, and the detection recovery rate me