Loop-mediated isothermal amplification reaction primer group and kit for detecting toxoplasma gondii in cat feces and use method of loop-mediated isothermal amplification reaction primer group

The invention relates to the technical field of parasites amplification detection. The invention provides a loop-mediated isothermal amplification reaction primer group for detecting toxoplasma gondii in cat feces, a kit and a use method of the kit. The single oocyst of the toxoplasma gondii in the cat excrement is detected through the visual LAMP method, the detection efficiency of amplification detection is improved, the sensitivity and specificity of toxoplasma gondii detection are improved, and meanwhile the detection method is also beneficial to application and popularization in primary laboratories and field detection. 本发明涉及寄生虫扩增检测技术领域。本发明提供了一种检测猫粪中弓形虫的环介导等温扩增反应引物组、试剂盒及其使用方法。本发明通过所述可视化LAMP方法来检测猫粪便中的弓形虫单个卵囊，不仅提高了扩增检测的检测效率，还提高了对弓形虫的检测的敏感性和特异性，同时本发明所述检测方法也有利于在基层实验室和现场检测中的推广应用。