Kit and method for rapidly detecting vibrio tower, vibrio mediterraneus and vibrio harveyi

The invention provides a kit and a method for rapidly detecting vibrio tower, vibrio mediterraneus and vibrio harveyi. The method comprises the following steps: firstly, designing a series of primer probe combinations aiming at three pathogenic bacteria of vibrio tower, vibrio mediterraneus and vibrio harveyi, and carrying out multiple rounds of screening to find a triple amplification primer probe group with the highest detection sensitivity; meanwhile, an amplification system is optimized, and oligopeptide and DMSO are added into the A Buffer, so that the amplification efficiency is remarkably improved, the detection sensitivity and specificity are improved, 0.5 copies/mu L of samples can be detected at least, the specificity is good, and the detection time is only 10-20 minutes. The method is of great significance to rapid detection of tower-type vibrio, vibrio mediterraneus and vibrio harveyi, cutting off of propagation paths and reduction of the risk of industrial aquaculture of Babylonia areolata. 本发明提供