Preparation method of L-amino acid ligase and application of L-amino acid ligase in dipeptide synthesis

The invention provides a preparation method of L-amino acid ligase and application of the L-amino acid ligase in dipeptide synthesis. The synthesis method of the dipeptide comprises the step of carrying out a dipeptide synthesis reaction by using L-amino acid ligase of an amino acid sequence as shown in SEQ ID NO: 1, so as to obtain the dipeptide. The L-amino acid ligase disclosed by the invention can be used for synthesizing various functional dipeptides, is relatively wide in substrate spectrum and relatively high in synthesis efficiency, can be relatively well applied to industrial amplification, is low in cost and high in yield, and realizes real green chemistry. 本发明提供了一种L-氨基酸连接酶的制备方法及其在二肽合成上的应用。其中，二肽合成方法包括利用如SEQ ID NO：1所示的氨基酸序列的L-氨基酸连接酶进行二肽合成反应，得到二肽。利用本申请的L-氨基酸连接酶，能够进行多种功能二肽的合成，底物谱较广，且合成效率较高，能够较好地用于工业放大，成本低，收率高，实现了真正的绿色化学。