Brown planthopper NlA7 gene and application of dsRNA of brown planthopper NlA7 gene in prevention and treatment of brown planthopper

The invention discloses a brown planthopper NlA7 gene and application of dsRNA of the brown planthopper NlA7 gene to prevention and treatment of brown planthopper. The nucleotide sequence of the NlA7 gene is as shown in the 1642nd site to the 3003rd site of SEQ ID NO.1, and the amino acid sequence c...

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Hauptverfasser: LI YANFANG, LIANG QICHANG, YUAN LONGYU, ZHANG ZHENFEI, XIAO HANXIANG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a brown planthopper NlA7 gene and application of dsRNA of the brown planthopper NlA7 gene to prevention and treatment of brown planthopper. The nucleotide sequence of the NlA7 gene is as shown in the 1642nd site to the 3003rd site of SEQ ID NO.1, and the amino acid sequence coded by the NlA7 gene is as shown in SEQ ID NO.2. The dsRNA of the nilaparvata lugens NlA7 gene can inhibit the expression of the nilaparvata lugens NlA7 gene, reduce the feeding of the nilaparvata lugens to rice and reduce the survival rate of the nilaparvata lugens. The cDNA sequence of the NlA7 gene of the brown planthopper is obtained, the NlA7 gene of the brown planthopper is silenced by adopting an RNAi (Ribonucleic Acid Interference) technology of microinjection dsRNA (Ribonucleic Acid), so that the brown planthopper generates an antifeedant effect, and obviously, the dsRNA of the gene has an important application value in the field of prevention and treatment of the brown planthopper. 本发明公开了褐飞虱NlA7基因及其dsRNA