Construction method of calculus disease in-vitro model and animal model

The invention discloses a construction method of a calculus disease in-vitro and animal model. The method disclosed by the invention comprises the following step: incubating the NETs releaser or neutrophil and NETs releaser system in a mineralizing solution to obtain the in-vitro model of the calculus disease. The animal model is a model constructed by using a PADI4 gene knockout animal. The invention focuses on the effect of mutual regulation and control between host immunity and infected microorganisms in the biomineralization process of calculus by simulating the infection microenvironment of calculus diseases. The construction method provided by the invention does not need special equipment, has no complex operation, can greatly reduce the research cost, and can be used for high-throughput research. 本发明公开了一种结石类疾病体外和动物模型的构建方法。所公开的方法包括：将NETs释放物或中性粒细胞和NETs释放物体系在矿化液中孵育后得到结石类疾病体外模型。所述动物模型是利用PADI4基因敲除动物构建的模型。本发明通过模拟结石类疾病发生的感染微环境，关注了宿主免疫与感染微生物之间的相互调控在结石的生物矿化过程中的作用。本发明的构建方法不需要特殊设备，无复杂操作，可大幅降低研究成本，可用于高通量研究。