Domestication method of anaerobic methanogen strain resistant to high acid and high ammonia nitrogen

The invention discloses a domestication method of a high-acid-resistant and high-ammonia-nitrogen-resistant anaerobic methanogenic bacterium system, the bacterium system comprises syntrophism propionic acid oxidative cracking bacteria, syntrophism fatty acid oxidizing bacteria, acetic acid type meth...

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Hauptverfasser: CHEN SHASHA, GUI LUN, LONG YUN, KANG MEIHUA, CHEN LIUMENG, YU AN, WU QIANGJIAN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a domestication method of a high-acid-resistant and high-ammonia-nitrogen-resistant anaerobic methanogenic bacterium system, the bacterium system comprises syntrophism propionic acid oxidative cracking bacteria, syntrophism fatty acid oxidizing bacteria, acetic acid type methanogenic bacteria, hydrogen nutrition type methanogenic bacteria and the like, fermentation liquor of a normal biogas project is used as an inoculum, full-quantified manure, fresh pig manure and straw are used as mixed raw materials, and the high-acid-resistant and high-ammonia-nitrogen-resistant anaerobic methanogenic bacterium system is prepared. According to the method, anaerobic fermentation strains are domesticated by gradually increasing the concentration of volatile fatty acid and ammonia nitrogen in fermentation liquor through a gradient load increasing method, a methanogenic strain with high acid resistance, high ammonia nitrogen resistance, high load impact resistance and good biological enhancement stabi