Method for simply, conveniently and rapidly concentrating urease-producing bacteria and application of urease-producing bacteria

The invention discloses a method for simply, conveniently and rapidly concentrating urease-producing bacteria and application thereof, and aims to solve the problem of limitation of storage and transportation of a huge-volume bacteria solution in practical engineering application of a biomineralizat...

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Bibliographische Detailangaben
Hauptverfasser: CHEN ZHIBO, LAI HANJIANG, CUI MINGJUAN, DING XINGZHI
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention discloses a method for simply, conveniently and rapidly concentrating urease-producing bacteria and application thereof, and aims to solve the problem of limitation of storage and transportation of a huge-volume bacteria solution in practical engineering application of a biomineralization technology. The method comprises the following steps: 1) adding a solution containing calcium ions into a bacterial liquid, and concentrating the bacterial liquid by a flocculation deposition method; and 2) transporting the concentrated bacterial liquid to a construction site, diluting the concentrated bacterial liquid to the original concentration with tap water, adjusting the pH value of the concentrated bacterial liquid to weak acidity, and mixing the diluted concentrated bacterial liquid with a cementing liquid to prepare a biomineralization treatment liquid for reinforcing a foundation. According to the method, calcium ions are added into the urease-producing bacterium liquid to concentrate the bacterium l