Image-based single molecule immune array analyzer and method thereof

The invention discloses an image-based single-molecule immune array analyzer and a method thereof, which are characterized in that in the analysis process of a single-molecule immune array, after molecules combined with an antibody are subjected to fluorescence labeling, an image processing and analysis algorithm is used for accurately detecting and analyzing a single-molecule fluorescence marker; according to the method, a single-molecule fluorescent marker detection image is processed and segmented by using a coding-decoding structure, so that an interested region is extracted from background noise, and other interference factors are removed, thereby enhancing the definition and visualization effect of a molecular signal, and being beneficial to improving the accuracy and reliability of single-molecule immune array analysis. 本申请公开了一种基于图像的单分子免疫阵列分析仪及其方法，其在进行单分子免疫阵列的分析过程中，在对于与抗体结合的分子进行荧光标记后，使用图像处理和分析算法来进行单分子荧光标志物的准确检测和分析，并利用编码-解码结构来完成单分子荧光标志物检测图像的处理和分割，以将感兴趣的区域从背景噪声中提取出来，并去除其他干扰因素，从而增强分子信号的清晰度和可视化效果，有助于提高单分子免