Typical mad cattle disease and atypical mad cattle disease distinguishing method

The invention relates to the technical field of virus detection, and particularly discloses a typical mad cattle disease and atypical mad cattle disease distinguishing method which specifically comprises the following steps: S1, sampling a sample; s2, sample cleaning; s3, sample cracking; s4, digestion treatment; s5, vertical electrophoresis; s6, identifying a primary antibody and a secondary antibody; s7, performing imaging detection; the prion disease is animal infectious spongiosis caused by protein conformation change, and three electrophoresis immunoblotting hybridization maps have special diagnostic significance through electrophoresis experiments, so that whether the mad cattle disease is typical or atypical is quickly distinguished. 本发明涉及病毒检测技术领域，具体公开了一种典型疯牛病和非典型疯牛病的区分方法，具体包括以下步骤：S1、样品取样；S2、样品清理；S3、样品裂解；S4、消化处理；S5、垂直电泳；S6、一抗、二抗识别；S7、成像检测；本发明通过朊病毒病是一种蛋白质构象发生改变而引起的动物传染性海绵样脑病，通过电泳实验，使三条电泳免疫印迹杂交图谱具有特殊诊断意义，进而快速区分疯牛病呈典型或者为非典型。