Method for accurately detecting sample size of quantitative loop of chromatographic instrument

The invention discloses a method for accurately detecting the sample size of a quantitative loop of a chromatograph. The method comprises the following steps: S1, preparing raw materials: coenzyme Q10, methanol and absolute ethyl alcohol; s2, preparation of a reference substance solution: taking coenzyme Q10, adding absolute ethyl alcohol, shaking and dissolving in a water bath state, cooling, adding the dissolved solution into a measuring flask, adding absolute ethyl alcohol, shaking uniformly again, and preparing the reference substance solution; the whole detection is not influenced by a chromatographic instrument and the model of the quantitative loop, the detection is carried out only aiming at the sample size of the quantitative loop and whether the sample size of the quantitative loop can influence the detection of the chromatographic instrument, the application range is larger, the whole data is sufficient, the coverage range is wider, the comprehensive data feedback can be carried out on the quantita