Dual-channel microscopic camera and particle image fusion method

The invention discloses a two-channel microscopic camera and particle image fusion method, which comprises the following steps: when a sample to be detected is detected to be placed at an imaging position, calling a two-color correction light source to irradiate the sample to be detected and acquiri...

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Bibliographische Detailangaben
Hauptverfasser: SHI ZHENZHI, KONG HANMING, NIE JIANGLONG, ZHOU WENJING, YANG SHIJING
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention discloses a two-channel microscopic camera and particle image fusion method, which comprises the following steps: when a sample to be detected is detected to be placed at an imaging position, calling a two-color correction light source to irradiate the sample to be detected and acquiring a bright field image and a fluorescence image, respectively extracting identification feature coordinates in the bright field image and the fluorescence image, calculating a geometric transformation coefficient corresponding to the fluorescence image according to the identification feature coordinates, when it is detected that the to-be-detected sample comprises particles, calling a double-color correction light source and a fluorescence excitation light source to irradiate the to-be-detected sample to obtain a bright field particle image and a fluorescence particle image, correcting the fluorescence particle image according to the geometric transformation coefficient to obtain a fluorescence correction image, a