CEA detection-based two-dimensional material biosensor as well as preparation method and application thereof

The invention discloses a two-dimensional material biosensor based on CEA detection and a preparation method and application thereof, and belongs to the technical field of two-dimensional material biosensing. According to the two-dimensional material biosensor based on CEA detection, a chemical vapor deposition method is utilized to grow a single-layer WS2 crystal onto a Si/SiO2 substrate, a wet transfer method is utilized to transfer the single-layer WS2 crystal onto an ITO substrate, then bis (trifluoromethane) sulfonimide is utilized to treat the single-layer WS2 crystal, and surface and interface chemical modification and Anti-CEA probe protein fixation are performed to obtain the biosensor. The prepared single-layer WS2 crystal is easy to grow, uniform in fluorescence and good in performance, and the biosensor has the advantages of being simple in process, high in sensitivity, low in detection limit, free of biomolecule marks and the like when used for detecting carcino-embryonic antigens. 本发明一种基于CEA检测的二