Construction method of genetic transformation system of citrus plants

The invention provides a construction method of a genetic transformation system of citrus plants, and belongs to the technical field of genetic transformation. A construction method of a genetic transformation system of citrus plants comprises the following steps: transforming a recombinant vector c...

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Bibliographische Detailangaben
Hauptverfasser: WANG MIN, SUN DEMING, MA HAIJIE, GU MIAOFENG, JIA ZHAOHUI, LI HANGFEI, HUANG SUYA, JIANG HUANGHUANG, ZHENG WEIWEI, MENG XINYUE, LIU NINGGE
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a construction method of a genetic transformation system of citrus plants, and belongs to the technical field of genetic transformation. A construction method of a genetic transformation system of citrus plants comprises the following steps: transforming a recombinant vector containing a target gene into an agrobacterium competent state to obtain recombinant agrobacterium; the method comprises the following steps: putting the base part of a branch or a leaf of a citrus plant into a bacterium solution of the recombinant agrobacterium, standing for 15-30 minutes in a vacuum state to obtain a branch or a leaf infected with the agrobacterium, then carrying out dark treatment on the branch or the leaf for 2 days, and then inserting the branch infected with the agrobacterium into a substrate for seedling culture to obtain the citrus plant with a transformed target gene. By adopting the construction method provided by the invention, the citrus branches are easy to sprout and root, the infiltra