Preparation method of recombinant horseradish peroxidase isoenzyme C1A

The invention discloses a preparation method of a recombinant horseradish peroxidase isoenzyme C1A. According to the invention, a polynucleotide sequence suitable for expression in pichia pastoris is designed, and an expression strain capable of expressing a large amount of horse radish peroxidase C1A protein is obtained through screening; the invention also develops a process method for producing the recombinant horse radish peroxidase C1A based on the sequence, the method is based on a eukaryotic system, the culture expression quantity is large, and the expression quantity can reach 23U/mL when induction is carried out for 60-120h; the method has the advantages of high stability, favorable multiple expression reproducibility and favorable activity of the obtained product, and is suitable for large-scale preparation. 本申请公开了一种重组辣根过氧化物酶同工酶C1A的制备方法。本申请中设计了适宜在毕赤酵母中表达的多核苷酸序列，并筛选得到可大量表达辣根过氧化物酶C1A蛋白的表达菌株；本申请还基于上述序列开发了生产重组辣根过氧化物酶C1A的工艺方法，该方法基于真核体系，培养表达量大，在诱导60-120h时，表达量可达到23U/mL；稳定性高，多次表达复现性良好，得到的产物活性好，适宜规模制备。