Isoeugenol monooxygenase mutant IEM-F305W-L470E, engineering bacteria and application

Isoeugenol monooxygenase mutant IEM-F305W-L470E, engineering bacteria and application

The invention discloses an isoeugenol monooxygenase mutant IEM-F305W-L470E, an engineering bacterium and application of the isoeugenol monooxygenase mutant IEM-F305W-L470E, and belongs to the technical field of biology. The isoeugenol monooxygenase gene is derived from Pseudomonas putida, the nucleo...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: YU TIANCHI, FENG ZHIBIN, FANG MEIFANG, ZHANG JUAN, WANG DONGYANG, CHEN ZHENG
Format: Patent
Sprache:chi ; eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The invention discloses an isoeugenol monooxygenase mutant IEM-F305W-L470E, an engineering bacterium and application of the isoeugenol monooxygenase mutant IEM-F305W-L470E, and belongs to the technical field of biology. The isoeugenol monooxygenase gene is derived from Pseudomonas putida, the nucleotide sequence of the isoeugenol monooxygenase gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the isoeugenol monooxygenase gene is as shown in SEQ ID NO: 2. The mutant is obtained by carrying out single mutation or multi-site mutation on the 305th site and the 470th site of an amino acid sequence as shown in SEQ ID NO: 2. According to the isoeugenol monooxygenase constructed by the invention and an engineering strain, the enzyme activity of a screened mutant is improved by 634.6% at most compared with the original enzyme activity, the isoeugenol monooxygenase is used for enzymatic synthesis of p-hydroxybenzaldehyde, the product concentration can reach 2.48 g/L at most, and the conversion rate can r