Real-time fluorescent PCR (Polymerase Chain Reaction) detection primer group and kit for brown rot of soybean stems and application of real-time fluorescent PCR detection primer group

The invention discloses a real-time fluorescent PCR (polymerase chain reaction) detection primer group and a kit for brown rot pathogen of soybean stems and application of the real-time fluorescent PCR detection primer group and the kit. The primer group comprises sequences as shown in SEQ ID NO.1-3. The kit comprises the primer group, a reaction solution A, a blank control and a positive control. The primer group disclosed by the invention has the advantages that the primer group has relatively high specificity and sensitivity in detection of the brown rot pathogen of soybean stems, and the detection result is accurate, efficient and reliable. 本发明公开了一种大豆茎褐腐病菌实时荧光PCR检测引物组、试剂盒及应用。引物组包括SEQ ID NO.1～3所示序列，试剂盒包括所述引物组、反应液A、空白对照、阳性对照。本发明的优点包括：在检测大豆茎褐腐病菌中，引物组具有较高的特异性和灵敏度、检测结果准确高效可靠。