Recombinant hepatitis B vaccine immunogenicity evaluation method and constructed cell strain

The invention relates to a recombinant hepatitis B vaccine immunogenicity evaluation method and a constructed cell strain. On one hand, the invention provides a method for constructing a Huh-7-HBsAg cell strain capable of stably expressing a hepatitis B surface antigen (HBsAg) gene, and the method comprises the following steps: constructing a plasmid vector containing a hepatitis B surface antigen HBsAg gene sequence, carrying out lentivirus packaging by using an HEK 293FT cell, and infecting a wild type Huh-7 cell strain by using a packaged gene overexpression lentivirus pHS-AVC-0612, so as to obtain the Huh-7-HBsAg cell strain capable of stably expressing the hepatitis B surface antigen HBsAg gene. And finally obtaining the Huh-7-HBsAg cell strain which is the stably transfected strain of the HBsAg overexpression cell line. According to the invention, the constructed cell strain is used for evaluating the immunogenicity of the recombinant hepatitis B vaccine for the first time, and a new method and a cell m