Preparation method and application of recombinant feline herpesvirus of new coronavirus

The invention discloses a preparation method and application of a recombinant feline herpesvirus of a new coronavirus, and the preparation method of the recombinant feline herpesvirus of the new coronavirus comprises the following steps: carrying out double enzyme digestion on a plasmid pUC19 by using XbaI and HindIII to obtain a linearized pUC19 plasmid fragment; a left arm and a right arm are amplified from FHV-1 virus genome DNA, a plasmid pUC-RBD is used as a template to amplify an RBD gene of SARS-CoV-2, and a pT-EGFP plasmid is used as a template to amplify a CMV gene promoter, a 2A self-cleavage polypeptide, an EGFP ORF and an SV40polyA signal; the left arm, the CMV gene promoter and the RBD gene are connected to obtain LgI-CMV-RBD, and the 2A self-cleavage polypeptide, the EGFP ORF and the SV40polyA are in signal connection to obtain the 2A-EGFP-SV40polyA-RgE. The vaccine prepared by the invention has good immunogenicity on a cat model, and can induce an organism to generate strong immune response. 本发