CrRNA and CRISPR-Cas system for detecting monkey pox virus and application

The invention provides CrRNA (Ribose Nucleic Acid) for detecting monkey pox virus. The CrRNA comprises Cas12a-crRNA and Cas13a-crRNA, wherein the Cas12a-crRNA comprises Cas13a- The base sequence of the Cas12a-crRNA is as shown in SEQ ID NO.1, and the Cas12a-crRNA is used for specific detection of th...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	LIN CHUNHUI, TANG HAO, XU HUAMING, ZHOU JING, HU XINYI, SHEN JILU, LIU RUNDE, JIANG TONG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The invention provides CrRNA (Ribose Nucleic Acid) for detecting monkey pox virus. The CrRNA comprises Cas12a-crRNA and Cas13a-crRNA, wherein the Cas12a-crRNA comprises Cas13a- The base sequence of the Cas12a-crRNA is as shown in SEQ ID NO.1, and the Cas12a-crRNA is used for specific detection of the monkey pox virus F3L gene; the base sequence of the Cas13a-crRNA is shown as SEQ ID NO.2, and the Cas13a-crRNA is used for specific detection of the monkey pox virus B6R gene. The invention also provides a CRISPR-Cas system and a method for rapidly detecting the monkey pox virus based on the CRISPR. Meanwhile, the invention also provides an application of the CRISPR-Cas system in preparation of a monkey pox virus detection kit. The kit has the advantages that the kit is suitable for rapid and convenient diagnosis of monkey pox virus infected patients under various medical conditions. 本发明提供了一种用于猴痘病毒检测的CrRNA，包括Cas12a-crRNA和Cas13a-crRNA；所述Cas12a-crRNA的碱基序列如SEQ ID NO.1所示，用于猴痘病毒F3L基因的特异性检测；所述Cas13a-crRNA的碱基序列如SEQ ID N