Kit and method for rapidly preparing filamentous fungus PCR template

The invention discloses a kit and a method for rapidly preparing a filamentous fungus PCR template. The method comprises the following steps: selecting a filamentous fungus colony with the age of 1-2 days, and picking a lawn; adding a lysate into the lawn, grinding in a grinding pestle, standing at 25-37 DEG C for 5-30 minutes, and taking supernate to obtain the filamentous fungus PCR template. The lysate is prepared from 0.1 mol/L to 1 mol/L of NaOH, 0.1 mol/L to 0.5 mol/L of LTris-HCl, 0.01 mol/L to 0.05 mol/L of LEDTA (Ethylene Diamine Tetraacetic Acid), 0.01 mol/L to 0.1 mol/L of CaCl2 and 0.1 percent to 0.5 percent of triton According to the method, operation such as washing and purification is not needed, the process is simple, the PCR template can be obtained only in 5 min at the soonest, the time of a molecular modification basic experiment of genome extraction is greatly shortened, and the filamentous fungus gene editing efficiency is greatly improved. 本申请公开了一种快速制备丝状真菌PCR模板的试剂盒和方法。本申请取菌龄1-2d的丝状真菌菌落，挑