Preparation method of recombinant deoxyribonuclease I

The invention discloses a preparation method of recombinant deoxyribonuclease I. The preparation method comprises the following steps: preparing recombinant deoxyribonuclease I; the preparation method of the deoxyribonuclease I, provided by the invention, shows excellent stability in process amplification, is relatively high in yield and is very suitable for industrial production; the expression system of deoxyribonuclease I constructed by the invention has a strict regulation promoter AOX1, is only induced by methanol, can secrete protein to a supernatant by using signal peptide, can greatly reduce the damage of toxic protein to host cells, and is very suitable for expression of DNase I. 本申请公开了一种重组脱氧核糖核酸酶I的制备方法。本申请中提供的脱氧核糖核酸酶I的制备方法，在工艺放大中表现出优异的稳定性，而且产量较高，十分适宜工业化生产；本申请构建的脱氧核糖核酸酶I的表达系统具有严格调控启动子AOX1，仅由甲醇诱导，且能利用信号肽将蛋白分泌至上清，可以极大减少有毒蛋白对宿主细胞的损害，非常适用于DNase I的表达。