Genetically engineered bacterium for producing succinic acid by using carbon dioxide as well as construction method and application of genetically engineered bacterium
The invention discloses a genetically engineered bacterium for producing succinic acid as well as a construction method and application of the genetically engineered bacterium, and belongs to the technical field of bioengineering. The invention also provides a construction method of the strain and a...
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Format: | Patent |
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Zusammenfassung: | The invention discloses a genetically engineered bacterium for producing succinic acid as well as a construction method and application of the genetically engineered bacterium, and belongs to the technical field of bioengineering. The invention also provides a construction method of the strain and a method for producing succinic acid by fermentation. An HCO3 transporter of blue-green algae is expressed in corynebacterium glutamicum, so that the corynebacterium glutamicum has the capacity of actively transporting HCO3 , and CO2 immobilization is enhanced through overexpression of a phosphoenolpyruvate carboxylase gene. HCO3 and CO2 which are transferred into cells are further immobilized in time through collaborative expression of transfer and immobilization genes, so that the CO2 utilization efficiency of recombinant engineering bacteria is increased, the yield and yield of succinic acid are improved, and the method has industrial application prospects.
本发明公开了一种产琥珀酸的基因工程菌及其构建方法与应用,属于生物工程技术领域;本发明还提供 |
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