Lactobacillus plantarum and application thereof in relieving constipation
The invention discloses lactobacillus plantarum HM-22, the preservation number of the lactobacillus plantarum HM-22 is CCTCC (China Center For Type Culture Collection) NO: M2023282, and the preservation number of the lactobacillus plantarum HM-22 is CCTCC NO: M2023282. The separation method comprise...
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creator | ZHANG RUI JIANG SHUJUAN QIAN FANG MOU GUANGQING |
description | The invention discloses lactobacillus plantarum HM-22, the preservation number of the lactobacillus plantarum HM-22 is CCTCC (China Center For Type Culture Collection) NO: M2023282, and the preservation number of the lactobacillus plantarum HM-22 is CCTCC NO: M2023282. The separation method comprises the following steps: uniformly mixing a collected Xinjiang traditional fermented yoghurt sample, carrying out gradient dilution, coating on an agar culture medium, carrying out anaerobic culture at 37 DEG C for 48 hours, taking a single colony, and carrying out streak purification on the agar culture medium to obtain a pure culture. According to the method, the excrement water content and the small intestine propulsion rate of constipation mice can be remarkably improved, and the first-grain black discharging time is shortened by about 30% compared with that of the constipation mice; the concentration of excitatory gastrointestinal regulatory peptide and 5-HT is obviously increased, the concentration of propionic |
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The separation method comprises the following steps: uniformly mixing a collected Xinjiang traditional fermented yoghurt sample, carrying out gradient dilution, coating on an agar culture medium, carrying out anaerobic culture at 37 DEG C for 48 hours, taking a single colony, and carrying out streak purification on the agar culture medium to obtain a pure culture. According to the method, the excrement water content and the small intestine propulsion rate of constipation mice can be remarkably improved, and the first-grain black discharging time is shortened by about 30% compared with that of the constipation mice; the concentration of excitatory gastrointestinal regulatory peptide and 5-HT is obviously increased, the concentration of propionic</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; HUMAN NECESSITIES ; HYGIENE ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230811&DB=EPODOC&CC=CN&NR=116574648A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230811&DB=EPODOC&CC=CN&NR=116574648A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ZHANG RUI</creatorcontrib><creatorcontrib>JIANG SHUJUAN</creatorcontrib><creatorcontrib>QIAN FANG</creatorcontrib><creatorcontrib>MOU GUANGQING</creatorcontrib><title>Lactobacillus plantarum and application thereof in relieving constipation</title><description>The invention discloses lactobacillus plantarum HM-22, the preservation number of the lactobacillus plantarum HM-22 is CCTCC (China Center For Type Culture Collection) NO: M2023282, and the preservation number of the lactobacillus plantarum HM-22 is CCTCC NO: M2023282. The separation method comprises the following steps: uniformly mixing a collected Xinjiang traditional fermented yoghurt sample, carrying out gradient dilution, coating on an agar culture medium, carrying out anaerobic culture at 37 DEG C for 48 hours, taking a single colony, and carrying out streak purification on the agar culture medium to obtain a pure culture. 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The separation method comprises the following steps: uniformly mixing a collected Xinjiang traditional fermented yoghurt sample, carrying out gradient dilution, coating on an agar culture medium, carrying out anaerobic culture at 37 DEG C for 48 hours, taking a single colony, and carrying out streak purification on the agar culture medium to obtain a pure culture. According to the method, the excrement water content and the small intestine propulsion rate of constipation mice can be remarkably improved, and the first-grain black discharging time is shortened by about 30% compared with that of the constipation mice; the concentration of excitatory gastrointestinal regulatory peptide and 5-HT is obviously increased, the concentration of propionic</abstract><oa>free_for_read</oa></addata></record> |
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subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE |
title | Lactobacillus plantarum and application thereof in relieving constipation |
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