Lactobacillus plantarum and application thereof in relieving constipation

The invention discloses lactobacillus plantarum HM-22, the preservation number of the lactobacillus plantarum HM-22 is CCTCC (China Center For Type Culture Collection) NO: M2023282, and the preservation number of the lactobacillus plantarum HM-22 is CCTCC NO: M2023282. The separation method comprise...

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Hauptverfasser: ZHANG RUI, JIANG SHUJUAN, QIAN FANG, MOU GUANGQING
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses lactobacillus plantarum HM-22, the preservation number of the lactobacillus plantarum HM-22 is CCTCC (China Center For Type Culture Collection) NO: M2023282, and the preservation number of the lactobacillus plantarum HM-22 is CCTCC NO: M2023282. The separation method comprises the following steps: uniformly mixing a collected Xinjiang traditional fermented yoghurt sample, carrying out gradient dilution, coating on an agar culture medium, carrying out anaerobic culture at 37 DEG C for 48 hours, taking a single colony, and carrying out streak purification on the agar culture medium to obtain a pure culture. According to the method, the excrement water content and the small intestine propulsion rate of constipation mice can be remarkably improved, and the first-grain black discharging time is shortened by about 30% compared with that of the constipation mice; the concentration of excitatory gastrointestinal regulatory peptide and 5-HT is obviously increased, the concentration of propionic