In-process verification of pH probe calibration status

Automated low pH virus inactivation systems and methods include adding an elution poke with an acid to a first container. Once the first container pH probe measures a sufficiently low pH, the pooling is transferred to the second container, at which time the pH is checked again, and the pooling is maintained for a period of time sufficient to reduce the virus concentration to a safe level, and neutralized, filtered and transferred to the third container. Meanwhile, the first container is filled with a buffer of known pH, which is examined for readings from the first container pH probe to determine whether recalibration is required. After transferring the pooling to the third container, the second container is filled with a buffer of known pH, which is examined for readings from a second container pH probe to determine whether recalibration is required. The process is repeated when a buffer of known pH is poured out and a new elution collection is added to the first container. 自动化低pH病毒灭活系统和方法包括将洗脱汇集物伴随酸添加至第一容器中