Detection method for rapidly detecting content of tryptophan based on chemical derivatization-surface enhanced Raman technology

Detection method for rapidly detecting content of tryptophan based on chemical derivatization-surface enhanced Raman technology

The invention relates to the technical field of tryptophan detection, in particular to a method for rapidly detecting the content of tryptophan based on a chemical derivatization-surface enhanced Raman technology. A detection method for rapidly detecting the content of tryptophan based on a chemical...

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Hauptverfasser: YU LILI, WU GUOQIANG, WU GUOPING, TAN ZHIMEI, YAN XIANZI, HONG YANPING, ZENG PEI, GUAN QI, WANG CHUNRONG
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Sprache:chi ; eng
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INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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creator YU LILI
WU GUOQIANG
WU GUOPING
TAN ZHIMEI
YAN XIANZI
HONG YANPING
ZENG PEI
GUAN QI
WANG CHUNRONG
description The invention relates to the technical field of tryptophan detection, in particular to a method for rapidly detecting the content of tryptophan based on a chemical derivatization-surface enhanced Raman technology. A detection method for rapidly detecting the content of tryptophan based on a chemical derivatization-surface enhanced Raman technology comprises the following steps: (1) preparing tryptophan standard substance solutions with different concentrations; (2) carrying out derivatization treatment on the tryptophan standard substance solutions with different concentrations in the step (1) by using 4-diethylaminobenzaldehyde to obtain tryptophan standard substance derivative solutions; (3) carrying out Raman spectrum detection on the tryptophan standard substance derivative solution; and (4) drawing a Raman spectrum standard curve of the tryptophan standard derivative. The method has the following advantages: 1, the operation steps are effectively simplified, and the detection limit of tryptophan is reduc
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subjects INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
title Detection method for rapidly detecting content of tryptophan based on chemical derivatization-surface enhanced Raman technology
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