Detection method for rapidly detecting content of tryptophan based on chemical derivatization-surface enhanced Raman technology

The invention relates to the technical field of tryptophan detection, in particular to a method for rapidly detecting the content of tryptophan based on a chemical derivatization-surface enhanced Raman technology. A detection method for rapidly detecting the content of tryptophan based on a chemical...

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Hauptverfasser: YU LILI, WU GUOQIANG, WU GUOPING, TAN ZHIMEI, YAN XIANZI, HONG YANPING, ZENG PEI, GUAN QI, WANG CHUNRONG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to the technical field of tryptophan detection, in particular to a method for rapidly detecting the content of tryptophan based on a chemical derivatization-surface enhanced Raman technology. A detection method for rapidly detecting the content of tryptophan based on a chemical derivatization-surface enhanced Raman technology comprises the following steps: (1) preparing tryptophan standard substance solutions with different concentrations; (2) carrying out derivatization treatment on the tryptophan standard substance solutions with different concentrations in the step (1) by using 4-diethylaminobenzaldehyde to obtain tryptophan standard substance derivative solutions; (3) carrying out Raman spectrum detection on the tryptophan standard substance derivative solution; and (4) drawing a Raman spectrum standard curve of the tryptophan standard derivative. The method has the following advantages: 1, the operation steps are effectively simplified, and the detection limit of tryptophan is reduc