Rapid detection kit for bladder cancer

The invention discloses a rapid detection kit for bladder cancer. Comprising a primer and a probe for detecting the HIST1H4F gene and the SOX1-OT gene, and nucleotide sequences of the primer and the probe are shown as SEQ ID NO: 1-4, 7 and 8. The primer and the probe disclosed by the invention are high in amplification efficiency, strong in specificity and good in stability. The methylation sensitive restriction enzyme is an enzyme mixed solution formed by mixing RruI, HpaII and TaiI, so that the enzyme digestion efficiency is effectively improved, the occurrence of incomplete enzyme digestion is reduced, the occurrence of false positive results is reduced, and the accuracy of a detection result is ensured. BSA (bovine serum albumin) and glycerol are added into an enzyme digestion amplification buffer solution, so that the activity of sensitive restriction endonuclease and Taq DNA (deoxyribonucleic acid) polymerase is protected, and the enzyme digestion efficiency is improved. The detection time is shortened,