Kit for detecting pneumoconiosis susceptibility gene and application thereof

The invention provides a kit for detecting a pneumoconiosis susceptibility gene and application of the kit. The kit comprises a primer for detecting a pneumoconiosis susceptibility gene SNP site and a restriction enzyme corresponding to the SNP site, the SNP site of the pneumoconiosis susceptible ge...

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Bibliographische Detailangaben
Hauptverfasser: WANG CUNFEI, QIAO JINLIN, WANG SHUGUANG, CHEN YIRAN
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention provides a kit for detecting a pneumoconiosis susceptibility gene and application of the kit. The kit comprises a primer for detecting a pneumoconiosis susceptibility gene SNP site and a restriction enzyme corresponding to the SNP site, the SNP site of the pneumoconiosis susceptible gene is selected from one or more of the following components: rs16944, rs1800796, rs4073 or rs562047, and the SNP site of the pneumoconiosis susceptible gene is selected from one or more of the following components; the primer is selected from one or more of the following primers with sequences as shown in SEQ ID NO: 1-8. One or more of SNP loci rs16944, rs1800796, rs4073 or rs562047 is/are subjected to pneumoconiosis susceptibility gene locus detection by utilizing the primers and related restriction endonucleases in the kit disclosed by the invention, and whether a sample to be detected has the pneumoconiosis susceptibility gene locus or not can be quickly and accurately obtained through an RFLP-PCR (Restriction F