Method for improving alternaria alternata resistance of tobacco plants and application
The invention provides a method for improving the brown spot resistance of a tobacco plant on the first aspect, and the method comprises the following steps: NtCCoAOMT6, NtCCoAOMT6L and NtCAD14 genes in tobacco are knocked out at fixed points by utilizing a CRISPR/CAS9 gene editing system, and the s...
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| creator | WANG JIN CHEN JIANHUA YANG GUANGYU ZHANG CHENGMING ZHU LONG HU WEIYAO LE ZHIWEI LI JING ZHANG JIANDUO KONG WEISONG XU YONG |
| description | The invention provides a method for improving the brown spot resistance of a tobacco plant on the first aspect, and the method comprises the following steps: NtCCoAOMT6, NtCCoAOMT6L and NtCAD14 genes in tobacco are knocked out at fixed points by utilizing a CRISPR/CAS9 gene editing system, and the sequences of the NtCCoAOMT6, NtCCoAOMT6L and NtCAD14 genes are respectively shown as SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3. The invention further provides a method for improving the brown spot resistance of the tobacco plant on the first aspect. The invention further provides application of the flue-cured tobacco leaves of the alternaria alternata resistant tobacco plants in cigarette combustion in the first aspect. The tobacco germplasm obtained by the invention has better resistance to tobacco brown spot. Cigarettes prepared from the tobacco leaves are good in combustion performance, the CO content is obviously low, and the tobacco harmfulness is reduced.
本发明第一方面提供了一种提升烟草植株抗赤星病的方法，其包括以下步骤：利用CRISPR/CAS9基因编辑系统定点敲 |
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本发明第一方面提供了一种提升烟草植株抗赤星病的方法，其包括以下步骤：利用CRISPR/CAS9基因编辑系统定点敲</description><language>chi ; eng</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; BEER ; BIOCHEMISTRY ; CHEMISTRY ; CIGARETTES ; CIGARS ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FISHING ; FORESTRY ; HUMAN NECESSITIES ; HUNTING ; MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING ORCHEWING ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; NEW PLANTS OR PROCESSES FOR OBTAINING THEM ; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SMOKERS' REQUISITES ; SNUFF ; SPIRITS ; TOBACCO ; TRAPPING ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230808&DB=EPODOC&CC=CN&NR=116555330A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230808&DB=EPODOC&CC=CN&NR=116555330A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>WANG JIN</creatorcontrib><creatorcontrib>CHEN JIANHUA</creatorcontrib><creatorcontrib>YANG GUANGYU</creatorcontrib><creatorcontrib>ZHANG CHENGMING</creatorcontrib><creatorcontrib>ZHU LONG</creatorcontrib><creatorcontrib>HU WEIYAO</creatorcontrib><creatorcontrib>LE ZHIWEI</creatorcontrib><creatorcontrib>LI JING</creatorcontrib><creatorcontrib>ZHANG JIANDUO</creatorcontrib><creatorcontrib>KONG WEISONG</creatorcontrib><creatorcontrib>XU YONG</creatorcontrib><title>Method for improving alternaria alternata resistance of tobacco plants and application</title><description>The invention provides a method for improving the brown spot resistance of a tobacco plant on the first aspect, and the method comprises the following steps: NtCCoAOMT6, NtCCoAOMT6L and NtCAD14 genes in tobacco are knocked out at fixed points by utilizing a CRISPR/CAS9 gene editing system, and the sequences of the NtCCoAOMT6, NtCCoAOMT6L and NtCAD14 genes are respectively shown as SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3. The invention further provides a method for improving the brown spot resistance of the tobacco plant on the first aspect. The invention further provides application of the flue-cured tobacco leaves of the alternaria alternata resistant tobacco plants in cigarette combustion in the first aspect. The tobacco germplasm obtained by the invention has better resistance to tobacco brown spot. Cigarettes prepared from the tobacco leaves are good in combustion performance, the CO content is obviously low, and the tobacco harmfulness is reduced.
本发明第一方面提供了一种提升烟草植株抗赤星病的方法，其包括以下步骤：利用CRISPR/CAS9基因编辑系统定点敲</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>CIGARETTES</subject><subject>CIGARS</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING ORCHEWING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</subject><subject>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SMOKERS' REQUISITES</subject><subject>SNUFF</subject><subject>SPIRITS</subject><subject>TOBACCO</subject><subject>TRAPPING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNyrEKAjEMgOFbHER9h_gAgkepuxyKi07iesReqoGahDb4_C66O_3f8M-725n8qRNkrcAvq_pmeQAWpypYGX90hEqNm6MkAs3geseUFKygeAOUCdCscEJnlWU3y1garb5ddOvj4TqcNmQ6UjNMJOTjcOn7XYwxhO0-_PN8AGTfOhM</recordid><startdate>20230808</startdate><enddate>20230808</enddate><creator>WANG JIN</creator><creator>CHEN JIANHUA</creator><creator>YANG GUANGYU</creator><creator>ZHANG CHENGMING</creator><creator>ZHU LONG</creator><creator>HU WEIYAO</creator><creator>LE ZHIWEI</creator><creator>LI JING</creator><creator>ZHANG JIANDUO</creator><creator>KONG WEISONG</creator><creator>XU YONG</creator><scope>EVB</scope></search><sort><creationdate>20230808</creationdate><title>Method for improving alternaria alternata resistance of tobacco plants and application</title><author>WANG JIN ; CHEN JIANHUA ; YANG GUANGYU ; ZHANG CHENGMING ; ZHU LONG ; HU WEIYAO ; LE ZHIWEI ; LI JING ; ZHANG JIANDUO ; KONG WEISONG ; XU YONG</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN116555330A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>AGRICULTURE</topic><topic>ANIMAL HUSBANDRY</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>CIGARETTES</topic><topic>CIGARS</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FISHING</topic><topic>FORESTRY</topic><topic>HUMAN NECESSITIES</topic><topic>HUNTING</topic><topic>MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING ORCHEWING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</topic><topic>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SMOKERS' REQUISITES</topic><topic>SNUFF</topic><topic>SPIRITS</topic><topic>TOBACCO</topic><topic>TRAPPING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>WANG JIN</creatorcontrib><creatorcontrib>CHEN JIANHUA</creatorcontrib><creatorcontrib>YANG GUANGYU</creatorcontrib><creatorcontrib>ZHANG CHENGMING</creatorcontrib><creatorcontrib>ZHU LONG</creatorcontrib><creatorcontrib>HU WEIYAO</creatorcontrib><creatorcontrib>LE ZHIWEI</creatorcontrib><creatorcontrib>LI JING</creatorcontrib><creatorcontrib>ZHANG JIANDUO</creatorcontrib><creatorcontrib>KONG WEISONG</creatorcontrib><creatorcontrib>XU YONG</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>WANG JIN</au><au>CHEN JIANHUA</au><au>YANG GUANGYU</au><au>ZHANG CHENGMING</au><au>ZHU LONG</au><au>HU WEIYAO</au><au>LE ZHIWEI</au><au>LI JING</au><au>ZHANG JIANDUO</au><au>KONG WEISONG</au><au>XU YONG</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for improving alternaria alternata resistance of tobacco plants and application</title><date>2023-08-08</date><risdate>2023</risdate><abstract>The invention provides a method for improving the brown spot resistance of a tobacco plant on the first aspect, and the method comprises the following steps: NtCCoAOMT6, NtCCoAOMT6L and NtCAD14 genes in tobacco are knocked out at fixed points by utilizing a CRISPR/CAS9 gene editing system, and the sequences of the NtCCoAOMT6, NtCCoAOMT6L and NtCAD14 genes are respectively shown as SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3. The invention further provides a method for improving the brown spot resistance of the tobacco plant on the first aspect. The invention further provides application of the flue-cured tobacco leaves of the alternaria alternata resistant tobacco plants in cigarette combustion in the first aspect. The tobacco germplasm obtained by the invention has better resistance to tobacco brown spot. Cigarettes prepared from the tobacco leaves are good in combustion performance, the CO content is obviously low, and the tobacco harmfulness is reduced.
本发明第一方面提供了一种提升烟草植株抗赤星病的方法，其包括以下步骤：利用CRISPR/CAS9基因编辑系统定点敲</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY CIGARETTES CIGARS COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING ORCHEWING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SMOKERS' REQUISITES SNUFF SPIRITS TOBACCO TRAPPING VINEGAR WINE |
| title | Method for improving alternaria alternata resistance of tobacco plants and application |
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