Method for improving alternaria alternata resistance of tobacco plants and application

The invention provides a method for improving the brown spot resistance of a tobacco plant on the first aspect, and the method comprises the following steps: NtCCoAOMT6, NtCCoAOMT6L and NtCAD14 genes in tobacco are knocked out at fixed points by utilizing a CRISPR/CAS9 gene editing system, and the sequences of the NtCCoAOMT6, NtCCoAOMT6L and NtCAD14 genes are respectively shown as SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3. The invention further provides a method for improving the brown spot resistance of the tobacco plant on the first aspect. The invention further provides application of the flue-cured tobacco leaves of the alternaria alternata resistant tobacco plants in cigarette combustion in the first aspect. The tobacco germplasm obtained by the invention has better resistance to tobacco brown spot. Cigarettes prepared from the tobacco leaves are good in combustion performance, the CO content is obviously low, and the tobacco harmfulness is reduced. 本发明第一方面提供了一种提升烟草植株抗赤星病的方法，其包括以下步骤：利用CRISPR/CAS9基因编辑系统定点敲