Acetolactate synthase III regulatory subunit coding gene ilvH, mutant thereof and application of coding gene ilvH in production of L-amino acid

The invention discloses an acetolactate synthase III regulatory subunit coding gene ilvH, a mutant thereof and application of the acetolactate synthase III regulatory subunit coding gene ilvH in production of L-amino acid. The invention provides an ilvH mutant protein or a fusion protein containing...

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Hauptverfasser:	CAI WEIWEI, ZHANG YING, BI GUODONG, WEI AIYING, MENG GANG, ZHAO CHUNGUANG, YANG LIPENG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPECIFIC USE OF COSMETICS OR SIMILAR TOILETPREPARATIONS SPIRITS THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses an acetolactate synthase III regulatory subunit coding gene ilvH, a mutant thereof and application of the acetolactate synthase III regulatory subunit coding gene ilvH in production of L-amino acid. The invention provides an ilvH mutant protein or a fusion protein containing the ilvH mutant protein. The ilvH mutant protein is a protein obtained by mutating the 47th amino acid residue of an acetolactate synthase III regulation subunit ilvH protein; experiments prove that in corynebacterium glutamicum, the yield of L-isoleucine can be increased by over-expression and point mutation of the corynebacterium glutamicum acetolactate synthase III regulation subunit coding gene ilvH; meanwhile, overexpression and point mutation of the ilvH gene in Escherichia coli can improve the yield of L-valine in Escherichia coli. 本发明公开了乙酰乳酸合酶III调控亚基编码基因ilvH和其突变体及其生产L-氨基酸中应用。本发明提供了ilvH突变蛋白或包含其的融合蛋白；所述ilvH突变蛋白为将乙酰乳酸合酶III调控亚基ilvH蛋白的第47位氨基酸残基进行突变，得到的蛋白；本发明的实验证明，在谷氨酸棒状杆菌中，谷氨酸棒状杆菌乙酰乳酸合酶III调控亚基编码基因ilvH的过表达及点突变能提高