Recombinant escherichia coli for fermentation production of L-valine

The invention discloses recombinant escherichia coli for fermentation production of L-valine. The recombinant Escherichia coli is obtained by knocking out an adhE gene in Escherichia coli for producing valine, knocking out the adhE gene and knocking out an ilvE (E) gene and inserting an ilvE (B) gene from bacillus subtilis at the same time, knocking out the adhE gene and a thiE gene and knocking out the ilvE (E) gene and inserting the ilvE (B) gene from bacillus subtilis at the same time, or knocking out the adhE gene. The engineering bacterium is obtained by knockout of a thiE gene and an mdh gene and insertion of an ilvE (B) gene from bacillus subtilis while the ilvE (E) gene is knocked out, the yield of L-valine can be increased by fermenting recombinant escherichia coli, and the engineering bacterium has important application value. 本发明公开了用于发酵生产L-缬氨酸的重组大肠杆菌。重组大肠杆菌为在生产缬氨酸的大肠杆菌中通过"敲除adhE基因"、"敲除adhE基因且敲除ilvE（E）基因同时插入来自枯草芽孢杆菌的ilvE（B）基因"、"敲除adhE基因和thiE基因且敲除ilvE（E）基因同时插入来自枯草芽孢杆菌的ilvE（B）基因"或"敲除adhE基因、thiE基因和mdh基