Schizochytrium limacinum and application thereof in fermentation production of double-low DHA (docosahexaenoic acid) algal oil
The invention discloses a strain of schizochytrium limacinum and application thereof in fermentation production of double-low DHA (docosahexaenoic acid) algal oil, and belongs to the technical field of bioengineering, and the preservation number of the schizochytrium limacinum is CGMCC (China Genera...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a strain of schizochytrium limacinum and application thereof in fermentation production of double-low DHA (docosahexaenoic acid) algal oil, and belongs to the technical field of bioengineering, and the preservation number of the schizochytrium limacinum is CGMCC (China General Microbiological Culture Collection Center) No.23203; the method comprises the following steps: activating schizochytrium limacinum with the seed age of 24 hours to obtain a schizochytrium limacinum seed solution, performing amplification culture on the schizochytrium limacinum seed solution through a first-stage seed tank and a second-stage seed tank, performing three-stage fermentation to obtain a schizochytrium limacinum fermentation solution, collecting the schizochytrium limacinum fermentation solution, centrifugally collecting thalli, drying, crushing, and extracting to obtain crude algae oil, the crude algae oil is subjected to nitrogen filling and oxygen isolation degumming, decoloration and deodorization |
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