Recombinant KOD polymerases

The invention relates to a recombinant KOD polymerase. The KOD polymerase mutant is a protein which is a protein with DNA polymerase activity and is obtained by modifying an amino acid residue of at least one of the 48 sites of 267, 326, 347, 349, 353, 375, 378, 379, 380, 385, 451, 452, 453, 457, 461, 465, 470, 474, 447, 478, 478, 478, 476, 476, 497, 514, 574, 584, 605, 610, 630, 665, 666, 667, 674, 676, 680, 682, 698, 707, 718, 723 and 729 in an amino acid sequence of KOD DNA polymerase GH78, and keeping other amino acid sequences unchanged; compared with the KOD DNA polymerase GH78, the recombinant DNA polymerase has the advantages of higher reaction rate, better catalytic efficiency, better affinity and the like in the aspect of catalysis, the reaction rate of the DNA polymerase in sequencing is increased, and the reaction reading length is increased. 一种重组型KOD聚合酶。KOD聚合酶突变体，为一种蛋白，如下蛋白：为将KOD DNA聚合酶GH78氨基酸序列中第267、326、347、349、353、375、378、379、380、385、451、452、453、454、457、461、465、470、474、477、478、479、480、482、484、4