Method for eliminating influence of gradient delay volume difference of different liquid chromatographs

The invention relates to the technical field of liquid chromatography, in particular to a method for eliminating influence of gradient delay volume difference of different liquid chromatographs, which comprises the following steps: measuring gradient delay volumes of different liquid chromatographs; setting a time fixed value; setting parameters of the gradient curve; wherein the initial section is a line segment parallel to a time axis, adjustment is performed according to gradient delay volumes of different liquid chromatographs, and the length of the parallel line segment is calculated. According to the method, no extra hardware device needs to be introduced, and the difference of chromatographic separation results caused by different gradient delay volumes of different instruments can be eliminated by changing the parameters of the gradient curve through the software or instrument operation panel of the existing liquid chromatograph. 本发明涉及液相色谱技术领域，尤其涉及一种消除不同液相色谱仪梯度延迟体积差异影响的方法，包括测定不同液相色谱仪的梯度延迟体积；设定一个时间固定值；