Primer group and kit for multi-target detection of blood flow infection

The invention belongs to the technical field of biological detection, and relates to a primer group and a kit for multi-target detection of blood flow infection, the primer group provided by the invention provides a plurality of targets, and can detect invasive infection caused by clinically common and high-incidence microorganisms with high sensitivity. The invention also provides a kit, which comprises the primer group and a PCR buffer solution, and the combination of the primer group and the PCR buffer solution can further improve the detection limit, reduce the loss of sample DNA during the subsequent sequencing detection of microorganisms, and improve the yield of the sample DNA. 本发明属于生物检测技术领域，涉及一种用于血流感染多靶标检测的引物组及试剂盒，本发明提供的引物组提供了多个靶标，可高灵敏度地检测临床常见和高发微生物造成的侵袭性感染。本发明还提供了一种试剂盒，包含引物组和PCR缓冲液，两者组合能进一步提高检出限，并减少了后续测序检测微生物时样本DNA的损失，提高了样本DNA的得率。