Application of calcium-dependent protein kinase gene GhCCAMK in verticillium wilt resistance of plants

The invention discloses an application of a calcium-dependent protein kinase gene GhCCAMK in verticillium wilt resistance of plants. Belongs to the technical field of gene engineering. The GhCCAMK gene is utilized to construct a VIGS plant expression vector to transform upland cotton TM-1, the obtained transgenic cotton shows verticillium wilt resistance after being inoculated with cotton verticillium wilt bacteria V991, and it is indicated that the GhCCAMK gene is highly related to the resistance of cotton verticillium wilt. According to the invention, an application foundation is laid for molecular mechanism research of verticillium wilt resistance and cultivation of new varieties of novel verticillium wilt-resistant plants, and new reference is provided for screening of new anti-disease genes and cultivation of resistant plants. 本发明公开了钙依赖蛋白激酶基因GhCCAMK在植物抗黄萎病中的应用。属于基因工程技术领域。本发明利用GhCCAMK基因构建VIGS植物表达载体转化陆地棉TM-1，获得的转基因棉花，在接种棉花黄萎病菌V991后表现出对黄萎病的抗性，说明GhCCAMK基因与棉花黄萎病的抗性高度相关。本发明为黄萎病抗性的分子机制研究、新的抗黄萎病植物新品种培育奠定了应用基础，同时