Chitinase with bacteriolytic activity as well as mutant and application of chitinase

The invention discloses chitinase with bacteriolytic activity as well as a mutant and application of the chitinase. The invention firstly provides chitinase with bacteriolytic activity, and the amino acid sequence of the chitinase is shown as SEQ ID No.1. According to the invention, chitinase is fur...

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Bibliographische Detailangaben
Hauptverfasser:	YAO BIN, HUANG HUOQING, WU NINGFENG, GUAN FEIFEI, TIAN JIAN, BAO YARU, LUO HUIYING, LIU XIAOQING
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PEST REPELLANTS OR ATTRACTANTS PLANT GROWTH REGULATORS PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses chitinase with bacteriolytic activity as well as a mutant and application of the chitinase. The invention firstly provides chitinase with bacteriolytic activity, and the amino acid sequence of the chitinase is shown as SEQ ID No.1. According to the invention, chitinase is further subjected to single site mutation of any one amino acid of Q8K, C28K, V121R, Q124E, D136E, N209R or K218R to obtain a single site mutant, and is subjected to double site mutation of C28K/D136E to obtain a double site mutant. The chitinase or each mutant provided by the invention has higher lysozyme activity, can be used for dissolving or destroying the bacterial wall of bacteria to realize a sterilization or bacteriostasis effect, and has application prospects in the aspects of killing harmful bacteria, preventing food corrosion and the like. 本发明公开了具有溶菌活性的几丁质酶及其突变体和应用。本发明首先提供了具有溶菌活性的几丁质酶，其氨基酸序列为SEQ ID No.1所示。本发明进一步对几丁质酶进行Q8K、C28K、V121R、Q124E、D136E、N209R或K218R中的任何一种氨基酸单位点突变获得单位点突变体以及进行C28K/D136E双位点突变获得双位点突变体。本发