Alcohol dehydrogenase ADH10C1 with improved thermal stability as well as gene and application thereof

The invention relates to ethanol dehydrogenase ADH10C1 with improved heat resistance as well as a coding gene and application thereof. The ethanol dehydrogenase ADH10C1 is obtained by mutation of the ethanol dehydrogenase ADH10 sequence SEQ ID NO.2 of the zygosaccharomyces rouxii CBS732, and the mut...

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Hauptverfasser:	LI FUWEI, WU PEIJUN, LUO JIANJIE, HUO MINGYUE
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HUMAN NECESSITIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to ethanol dehydrogenase ADH10C1 with improved heat resistance as well as a coding gene and application thereof. The ethanol dehydrogenase ADH10C1 is obtained by mutation of the ethanol dehydrogenase ADH10 sequence SEQ ID NO.2 of the zygosaccharomyces rouxii CBS732, and the mutation sites of the ethanol dehydrogenase ADH10 sequence SEQ ID NO.2 are G201C, G207C, P52C and E56C. The mutant can still maintain 80% of enzyme activity at a high temperature of 70 DEG C for 5 min, while ADH10 can only maintain 60% of enzyme activity at a temperature of 60 DEG C for 5 min; the optimal pH value of the mutant is 8.5, and the mutant has higher enzyme activity and good pH stability when the pH value is 7.5-10.5; the mutant has good thermal stability and protease resistance. According to the invention, the resource of the ethanol dehydrogenase gene is expanded, excellent ethanol dehydrogenase is provided for converting ethanol into acetaldehyde in the ethanol metabolism process, and the ethanol dehydro