Method for preserving autologous skull at ultralow temperature

The invention provides a method for preserving autologous skull at ultralow temperature, which belongs to the technical field of human tissue storage, and comprises the following steps of: preparing pseudo-ginseng/angelica sinensis extract by adopting a water extraction method, adding the pseudo-ginseng/angelica sinensis extract into polyvinyl alcohol-sodium alginate-polyacrylic acid hydrogel, repeatedly freezing and thawing, sterilizing by ultraviolet rays, freezing and drying to prepare modified hydrogel, and then removing tissues from skull flaps to obtain the skull. The preparation method comprises the following steps: cleaning blood stains, soaking in a glycerol solution, taking out, uniformly coating a layer of organ preserving fluid on the surface, carrying out ultraviolet sterilization to obtain a pretreated skull flap, pre-freezing the pretreated skull flap, adding into modified hydrogel, carrying out ultrasonic exhaust and staged cooling, and storing in a liquid nitrogen tank. The stored autologous