Flow detection system and method for protein

The invention discloses a flow type detection system and method of protein, the flow type detection system comprises a micro-fluidic chip, the micro-fluidic chip comprises a detection micro-channel, the detection micro-channel is configured to allow microspheres in a microsphere solution to pass thr...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: YAN JING, ZHOU FENGMAO, FANG WENLEI
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention discloses a flow type detection system and method of protein, the flow type detection system comprises a micro-fluidic chip, the micro-fluidic chip comprises a detection micro-channel, the detection micro-channel is configured to allow microspheres in a microsphere solution to pass through one by one, the surface of each microsphere is coupled with a protein capture antibody, and the detection micro-channel is configured to allow the microspheres to pass through the detection micro-channel one by one; the microspheres are subjected to mixed incubation with a sample and a fluorescent substance coupled antibody; the optical detection device is arranged above the micro-fluidic chip and is used for detecting the number of microspheres with fluorescence and the number of microspheres without fluorescence; wherein the optical detection device comprises a laser light source, a reflecting mirror, a dichroscope and a first lens which are arranged in sequence; the optical detection device further comprise