Application of ATAC-seq mediated precise targeted editing in rice disease resistance

The invention discloses application of ATAC-seq mediated precise targeted editing in rice disease resistance, and belongs to the technical field of gene editing. Eight sites are designed based on ATAC-seq and precise targets, four of the eight sites are located in a high ATAC-seq area, the other four target sites are located in a low ATAC-seq area, the working efficiency is improved, and precise editing of genes can be achieved. On the basis of ATAC-seq combined gene editing, the accuracy and specificity of original target design are improved, the method can be applied to screening and verification of rice disease-resistant genes, gene expression is accurately regulated and controlled, and the purpose of rice disease resistance is achieved. 本发明公开了ATAC-seq介导精准靶向编辑在水稻抗病中的应用，属于基因编辑技术领域。本发明基于ATAC-seq，精准靶点设计八个位点，其中四个位点在高ATAC-seq区域，另外四个靶位点位于低ATAC-seq区域，提升工作效率，可以实现基因的精准编辑。本发明基于ATAC-seq结合基因编辑，提高原先靶点设计的精准性以及特异性，可应用于水稻抗病基因的筛选和验证，并精准调控基因表达，实现水稻抗病的目的。