Morchella ISSR-PCR molecular marker method
The invention relates to the field of molecular biology, in particular to a morchella esculenta ISSR-PCR molecular marking method. The invention discloses a morchella esculenta ISSR-PCR molecular marking method. The method comprises the following steps: 1) extracting morchella esculenta genome DNA;...
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| creator | SUN YANFANG LU ZHONGHUA HU CHUANJIU ZHU XIQIANG HU ZHONGNAN YUAN WEIDONG LU NA SUN ZHIXIN JIANG JUANPING |
| description | The invention relates to the field of molecular biology, in particular to a morchella esculenta ISSR-PCR molecular marking method. The invention discloses a morchella esculenta ISSR-PCR molecular marking method. The method comprises the following steps: 1) extracting morchella esculenta genome DNA; 2) carrying out PCR (Polymerase Chain Reaction) amplification; and (3) carrying out electrophoresis detection, observation and photographing on a PCR amplification product. The method comprises the following steps of: establishing an initial data matrix, calculating a genetic correlation coefficient by using NTSYSpc2.1 software, and performing clustering analysis by using an average linkage method (UPGMA), wherein the number of the strips with amplified strips is 1, and the number of the strips without strips at the same position is 0, constructing the initial data matrix, and calculating the genetic correlation coefficient by using NTSYSpc2.1 software. The method disclosed by the invention is simple and convenient |
| format | Patent |
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The invention discloses a morchella esculenta ISSR-PCR molecular marking method. The method comprises the following steps: 1) extracting morchella esculenta genome DNA; 2) carrying out PCR (Polymerase Chain Reaction) amplification; and (3) carrying out electrophoresis detection, observation and photographing on a PCR amplification product. The method comprises the following steps of: establishing an initial data matrix, calculating a genetic correlation coefficient by using NTSYSpc2.1 software, and performing clustering analysis by using an average linkage method (UPGMA), wherein the number of the strips with amplified strips is 1, and the number of the strips without strips at the same position is 0, constructing the initial data matrix, and calculating the genetic correlation coefficient by using NTSYSpc2.1 software. The method disclosed by the invention is simple and convenient</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROCESSES USING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230801&DB=EPODOC&CC=CN&NR=116515969A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230801&DB=EPODOC&CC=CN&NR=116515969A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>SUN YANFANG</creatorcontrib><creatorcontrib>LU ZHONGHUA</creatorcontrib><creatorcontrib>HU CHUANJIU</creatorcontrib><creatorcontrib>ZHU XIQIANG</creatorcontrib><creatorcontrib>HU ZHONGNAN</creatorcontrib><creatorcontrib>YUAN WEIDONG</creatorcontrib><creatorcontrib>LU NA</creatorcontrib><creatorcontrib>SUN ZHIXIN</creatorcontrib><creatorcontrib>JIANG JUANPING</creatorcontrib><title>Morchella ISSR-PCR molecular marker method</title><description>The invention relates to the field of molecular biology, in particular to a morchella esculenta ISSR-PCR molecular marking method. The invention discloses a morchella esculenta ISSR-PCR molecular marking method. The method comprises the following steps: 1) extracting morchella esculenta genome DNA; 2) carrying out PCR (Polymerase Chain Reaction) amplification; and (3) carrying out electrophoresis detection, observation and photographing on a PCR amplification product. The method comprises the following steps of: establishing an initial data matrix, calculating a genetic correlation coefficient by using NTSYSpc2.1 software, and performing clustering analysis by using an average linkage method (UPGMA), wherein the number of the strips with amplified strips is 1, and the number of the strips without strips at the same position is 0, constructing the initial data matrix, and calculating the genetic correlation coefficient by using NTSYSpc2.1 software. 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The invention discloses a morchella esculenta ISSR-PCR molecular marking method. The method comprises the following steps: 1) extracting morchella esculenta genome DNA; 2) carrying out PCR (Polymerase Chain Reaction) amplification; and (3) carrying out electrophoresis detection, observation and photographing on a PCR amplification product. The method comprises the following steps of: establishing an initial data matrix, calculating a genetic correlation coefficient by using NTSYSpc2.1 software, and performing clustering analysis by using an average linkage method (UPGMA), wherein the number of the strips with amplified strips is 1, and the number of the strips without strips at the same position is 0, constructing the initial data matrix, and calculating the genetic correlation coefficient by using NTSYSpc2.1 software. The method disclosed by the invention is simple and convenient</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Morchella ISSR-PCR molecular marker method |
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