Morchella ISSR-PCR molecular marker method

The invention relates to the field of molecular biology, in particular to a morchella esculenta ISSR-PCR molecular marking method. The invention discloses a morchella esculenta ISSR-PCR molecular marking method. The method comprises the following steps: 1) extracting morchella esculenta genome DNA; 2) carrying out PCR (Polymerase Chain Reaction) amplification; and (3) carrying out electrophoresis detection, observation and photographing on a PCR amplification product. The method comprises the following steps of: establishing an initial data matrix, calculating a genetic correlation coefficient by using NTSYSpc2.1 software, and performing clustering analysis by using an average linkage method (UPGMA), wherein the number of the strips with amplified strips is 1, and the number of the strips without strips at the same position is 0, constructing the initial data matrix, and calculating the genetic correlation coefficient by using NTSYSpc2.1 software. The method disclosed by the invention is simple and convenient