Nucleic acid detection method based on deoxyribozyme enhanced nonlinear hybridization chain reaction

The invention relates to the field of nucleic acid amplification, in particular to a nucleic acid detection method based on deoxyribozyme enhanced nonlinear hybridization chain reaction. The invention provides application of deoxyribozyme and a nucleic acid molecule S-L in improvement of the reaction upper limit of a branched hybridization chain reaction, reduction of spontaneous amplification of the branched hybridization chain reaction and reduction of a background signal value and a kit of the deoxyribozyme and the nucleic acid molecule S-L. The method breaks through the bottleneck of the current nucleic acid molecule detection and imaging technology, realizes in-vitro ultrasensitive, rapid and simple detection of nucleic acid molecules, and has great clinical value for diagnosis of diseases and monitoring of pathogenic microorganisms. 本发明涉及核酸扩增领域，特别涉及一种基于脱氧核酶增强的非线性杂交链式反应的核酸检测方法。本发明提供了脱氧核酶和核酸分子S-L在提高分枝杂交链式反应的反应上限、减少分枝杂交链式反应的自发扩增、降低背景信号值中的应用及其试剂盒。其突破目前核酸分子检测及成像技术的瓶颈，实现核酸分子体外的超灵敏、快速、简便检测，对于疾病的诊断以及病原微生物的监测具有重