Phosphoglycerol oxidase as well as mutant, preparation method and application thereof

The invention discloses phosphoglycerol oxidase as well as a mutant, a preparation method and application thereof. In the application, the phosphoglycerol oxidase mutant is obtained by mutation of enterococcus phosphoglycerol oxidase, and the stability of the phosphoglycerol oxidase mutant is better than that of enterococcus phosphoglycerol oxidase or streptococcus agalactiae phosphoglycerol oxidase; based on a phosphoglycerol oxidase Escherichia coli expression system of genetic engineering, through strain source screening and synonymous codon preference optimization, the yield is high, and the obtained product is good in activity and stability. 本申请公开了一种磷酸甘油氧化酶及其突变体、制备方法和应用。本申请中，磷酸甘油氧化酶突变体，经肠球菌属磷酸甘油氧化酶突变获得，稳定性好于肠球菌属磷酸甘油氧化酶或无乳链球菌属磷酸甘油氧化酶；基于基因工程的磷酸甘油氧化酶大肠杆菌表达体系，经过菌种来源的筛选和同义密码子偏好性优化，产量高，所得产物活性好，稳定性好。